Wyszukano: Ekstrakcji+bia\\u0142ka

Producent: Merck Millipore (Novagen)

Opis: BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from <i>E. coli</i> without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

Numer katalogowy: (PIER89802)

Producent: Thermo Fisher Scientific

Opis: The I-PER Insect Cell Protein Extraction Reagent enables gentle and effective extraction of soluble protein from baculovirus-infected insect cells grown in suspension or monolayer culture. The baculovirus insect cell expression system is an efficient and popular system for production of recombinant (eukaryotic) proteins in cell culture. Proteins expressed in baculoviral systems can be used for structural analyses, biochemical assays and a variety of other applications. I-PER Reagent maintains functionality of extracted proteins and is directly compatible with downstream applications such as protein assays, Western blotting and 6xHis-tagged protein purification.

j.m.: 1 * 250 mL

Wyprzedaż

Producent: VARIAN

Opis: EnvirElut™ sorbents are specially designed for the extraction of a wide range of compounds from aqueous matrices. EnvirElut™ PAH and Pesticides are available in standard SPE straight barrel cartridges, which can be used on conventional vacuum manifolds such as the Vac Elut SPS 24.

Producent: VARIAN

Opis: Bond Elut™ Jr cartridges have top and bottom Luer fittings allowing easy sample processing when used in conjunction with standard SPE cartridges. Bond Elut™ LRC cartridges have a large reservoir above the sorbent bed and are suitable for use on any standard SPE vacuum manifold.

Producent: G-Biosciences

Opis: Tissue PE LB™ has been developed for extraction of total biologically active, soluble proteins from animal tissues. Tissue PE LB™ is based on an organic buffer and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Tissue PE LB™. Tissue PE LB™ has been tested for use with a wide variety of fresh and frozen animal tissues.

Producent: VARIAN

Opis: Native silica is generally regarded as the most polar SPE sorbent available. Bond Elut™ SI is particularly effective at separating compounds with a very similar structure. Applying the analytes in a non-polar solvent, then increasing the solvent polarity by increasing the concentration of a polar modifier, such as THF or ethyl acetate, delivers effective separations.

Producent: VARIAN

Opis: Bond Elut™ ENV, a PS/DVB polymer, is designed for the extraction of polar organic residues. It contains 125 μm spherical particles with a high degree of cross linking, advantageous for high volume, fast flow-through applications.

Producent: VARIAN

Opis: Alumina, like silica, is an extremely polar sorbent. The alumina surface tends to be slightly more stable under high pH conditions than unfunctionalised silica. The small particle size of the Bond Elut™ Alumina range ensures high extraction efficiency even when small bed masses are used.

Producent: Merck

Opis: LiChrolut® solid phase extraction (SPE) cartridges and accessories are manufactured to the highest standards. All SPE cartridges listed below are polypropylene unless otherwise stated. They are compatible with all automated SPE instruments and robots. Three important criteria are carefully controlled.

MSDS

Producent: Quantabio

Opis: Synthesis of cDNA and PCR amplification are carried out in the same tube without opening between procedures. These kits are optimised to deliver maximum RT-PCR efficiency and specificity, enabling unbiased co-amplification of low copy transcripts in the presence of higher copy reference genes. As a result the qScript™ One-Step qRT-PCR Kits offer improved sensitivity over other commercially available qPCR reagents. The qScript™ One-Step Master Mix maximises the activities of both reverse transcriptase and PCR enzymes, while minimising primer-dimer formation. High specificity is assured with AccuStart™ <i>Taq</i> DNA Polymerase, which is bound by monoclonal antibodies for superior hot-start. Upon heating to 95 °C, the antibodies denature irreversibly releasing fully active and unmodified <i>Taq</i> DNA polymerase. qScript™ One-Step qRT-PCR Kits are available for all Real-Time PCR instrument platforms, including those requiring normalisation with ROX reference dye.

Certyfikaty

Numer katalogowy: (786-180)

Producent: G-Biosciences

Opis: Mammalian Cell PE LB™ has been developed for extraction of total biologically active, soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents, phosphatase and protease inhibitors may be added into Mammalian Cell PE LB™. Mammalian Cell PE LB™ has been tested on a wide variety of mammalian cells and can be used for both suspension and adherent cells.

j.m.: 1 * 500 mL

Wyprzedaż

Producent: VARIAN

Opis: Bond Elut™ EWP is based upon standard particle size silica but with 500 Å pores to allow more efficient extraction of large molecules (>15000 MW), which are typically excluded from standard porosity silica phases.

Producent: VARIAN

Opis: Bond Elut AccuCAT cartridges are mixed bed SPE cartridges consisting of a strong cation exchange (SCX) and a strong anion exchange (SAX) sorbent packed into one bed. AccuCAT is effective for the extraction of acidic, basic and neutral analytes from urine and other biological samples. AccuCAT is particularly effective for catecholamine extraction from bio fluids.

Producent: VARIAN

Opis: Bond Elut™ CH is a mid-polarity sorbent that exhibits unique selectivities for certain analytes. When employed as a non-polar sorbent, CH has the approximate polarity of a C₂ sorbent. Bond Elut™ CH is often a good choice when non-polar sorbents such as C₁₈, C₈ or C₂ do not provide the desired selectivity.

Producent: Merck Millipore (Novagen)

Opis: BugBuster® Plus Lysonase™ includes BugBuster Protein Extraction Reagent and Lysonase Bioprocessing Reagent, an optimised, ready-to-use blend of rLysozyme™ and Benzonase® Nuclease in a single kit. This powerful combination of two reagents allows maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The BugBuster® and rLysozyme™ efficiently lyse the bacterial cells while the Benzonase® degrades nucleic acids to facilitate downstream processing. Requires 5 ml BugBuster and 10 µl Lysonase per 1 g cell paste. The kits provide sufficient reagents for protein extraction from 20 g or 100 g cell paste.

Producent: VARIAN

Opis: Bond Elut™ C2 is a fairly non-polar sorbent because of the short chain length of the functional group. C2 is often used during the process of method development if analytes are retained too strongly on a C8 or C18 phase. The polarity of C2 is slightly lower than a cyano phase for polar interactions.