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Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.


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Producent: ENZO LIFE SCIENCES
Opis: Antigen retrieval reagent is used to recover antigens masked by fixation in cross linking fixatives such as formalin.

Numer katalogowy: (786-244)
Producent: G-Biosciences
Opis: SilverOUT removes interfering silver ions from gel pieces.
j.m.: 1 * 100 Tests


Numer katalogowy: (DGA01)
Producent: G-Biosciences
Opis: The 'critical micelle concentration' (CMC) of a detergent varies with temperature, pH, ionic strength, detergent concentration, purity and presence of organic agents in the detergent. Using a large excess of detergent may pose problems during purification procedures or other downstream applications.
j.m.: 1 * 500 SZT


Numer katalogowy: (786-598)
Producent: G-Biosciences
Opis: Immobilised biotin resin is designed for the high affinity chromatography purifications of avidin, streptavidin and neutravidin protein. The resin consists of biotin coupled to 6% cross-linked agarose.
j.m.: 1 * 5 mL


Numer katalogowy: (786-793)
Producent: G-Biosciences
Opis: Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the endopeptidase activity to cleave Immunoglobulin G (IgG) molecules in the hinge region.
j.m.: 1 * 5 mL


Numer katalogowy: (786-681)
Producent: G-Biosciences
Opis: InGel™ Blue provides a complete set of reagents for the in-gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS). The kit is specifically designed for use with Coomassie® or fluorescent stained protein spots/bands.
j.m.: 1 * 1 KIT


Numer katalogowy: (786-241)
Producent: G-Biosciences
Opis: InGel™ provides a reliable method for the proteolytic digestion and subsequent extration of silver stained proteins in gel for subsequent analysis by mass spectrometry.
j.m.: 1 * 1 KIT


Producent: G-Biosciences
Opis: Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to β-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider range of pH, including lower acidic pHs. Protein-S-S-Reductant™ completely reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions. This ready to use solution is at a neutral pH and stabilised for long-term storage. Simply supplement Protein-S-S-Reductant™ in place of DTT or β-mercaptoethanol and boil the sample.

Producent: Thermo Fisher Scientific
Opis: The Pierce™ Universal Nuclease for Cell Lysis is ideal for a wide variety of applications where complete digestion of nucleic acids is needed when preparing cell lysates.
Numer katalogowy: (ENZOBMLKI1761250)
Producent: ENZO LIFE SCIENCES
Opis: FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates. Deacetylation of the FLUOR DE LYS® substrate sensitises it so that, in a second step, treatment with the FLUOR DE LYS® Developer II produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1X Developer dilution (e.g. nicotinamide for sirtuins).
j.m.: 1 * 1,25 mL


Numer katalogowy: (PIER90054)
Producent: Thermo Fisher Scientific
Opis: Glu-C protease is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications.
j.m.: 1 * 50 µG

MSDS


Numer katalogowy: (PIER90056)
Producent: Thermo Fisher Scientific
Opis: Chymotrypsin protease is a purified native endoproteinase validated for maximum activity and selectivity in proteomic applications.
j.m.: 1 * 100 µG


Numer katalogowy: (PIER90053)
Producent: Thermo Fisher Scientific
Opis: Asp-N protease is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications.
j.m.: 1 * 2 µG

MSDS


Numer katalogowy: (786-599)
Producent: G-Biosciences
Opis: Immobilised Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose. The resin allows for the purification of avidin, streptavidin and Neutravidin and their subsequent gentle elution using non denaturing elution buffers.
j.m.: 1 * 5 mL


Producent: G-Biosciences
Opis: The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research. A crucial step in using peptides as antigens is their coupling to carrier proteins. Peptides are designed to be good epitopes by themselves; however they fail to elicit a strong antibody response. The reason for this is that the epitopes are able to bind to the B-cells, but are too small to contain additional motifs required to associate with class II molecules and T-cell receptors. The carrier proteins, once coupled to the peptides, provide these additional motifs and generate a strong antibody response. All three carrier proteins are also offered as activated carrier proteins, named as ActiveHOOK™.

Producent: G-Biosciences
Opis: DetergentOUT™ Tween® is a spin column format detergent removal resin for polysorbate or Tween® detergents or surfactants.
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Zapasy tego artykułu są ograniczone, ale możliwe, że jest on dostępny w pobliskim magazynie. Upewnij się, że jesteś zalogowany na stronie, aby móc sprawdzić dostępność zapasów. Jeśli call ciągle się wyświetla i potrzebujesz pomocy, zadzwoń na 58 323 82 00.
Ten produkt jest ograniczony w dostępie i można go zamówić tylko przy użyciu zaaprobowanego konta. Jeśli potrzebujesz pomocy, napisz do Działu Prawnego VWR na tomasz.chrobak@pl.vwr.com
Do zakupu tego produktu może być potrzebna dodatkowa dokumentacja. Przedstawiciel VWR skontaktuje się z Tobą w razie potrzeby.
Ten produkt jest zablokowany na stronie internetowej. W celu zamówienia, skontaktuj się z Działem Obsługi Klienta.
Produkt oryginalny został wycofany. Dostępny jest wskazny zamiennik .
Wybrany produkt został wycofany - sprzedaż do wyczerpania zapasów. Dostępne zamienniki można znaleźć poprzez wpisanie powyższego numeru katalogowego VWR w okno wyszukiwarki. Jeśli potrzebujesz dalszej pomocy, prosimy o kontakt z Działem Doradztwa Produktowego pod numerem telefonu 58 3238 220.
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