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Wyszukano: Odczynniki do ekstrakcji białek

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.


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Numer katalogowy: (ENZOBMLKI1050300)
Producent: ENZO LIFE SCIENCES
Opis: FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS® Developer produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1x Developer dilution (e.g. Trichostatin A) for class I and II HDACs, nicotinamide for sirtuins).
j.m.: 1 * 300 µl

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Producent: G-Biosciences
Opis: The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research. A crucial step in using peptides as antigens is their coupling to carrier proteins. Peptides are designed to be good epitopes by themselves; however they fail to elicit a strong antibody response. The reason for this is that the epitopes are able to bind to the B-cells, but are too small to contain additional motifs required to associate with class II molecules and T-cell receptors. The carrier proteins, once coupled to the peptides, provide these additional motifs and generate a strong antibody response. All three carrier proteins are also offered as activated carrier proteins, named as ActiveHOOK™.

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Udoskonalenia w zakresie lokalizacji zapasów magazynowych

Producent: G-Biosciences
Opis: DetergentOUT™ Tween® is a spin column format detergent removal resin for polysorbate or Tween® detergents or surfactants.
Producent: G-Biosciences
Opis: OrgoSol DetergentOUT™ is suitable for removal of detergents from protein solutions, including hydrophobic protein solutions. Compatible with all detergent types, its performance is not dependent on the concentration of detergents in the solution. It is highly flexible and can be used to process small and large sample volumes.

Numer katalogowy: (PIER90051)
Producent: Thermo Fisher Scientific
Opis: Endoproteinase Lys-C protease is a highly purified native endoproteinase validated for maximum activity and stability in proteomic applications.
j.m.: 1 * 20 µG


Numer katalogowy: (786-428)
Producent: G-Biosciences
Opis: Adenine deaminase converts adenine to hypoxanthine. It is a highly purified recombinant enzyme protein supplied at a 1 µM concentration.
j.m.: 1 * 0,25 mL


Numer katalogowy: (PIER84841)
Producent: Thermo Fisher Scientific
Opis: The Digestion Indicator for Mass Spectrometry is a non-mammalian recombinant protein (26kDa) with five signature peptides for use in determining the digestion efficiency and reproducibility across multiple samples.
j.m.: 1 * 10 µG


Numer katalogowy: (PIER20343)
Producent: Thermo Fisher Scientific
Opis: Immobilised Pepsin agarose consists of an acidic endopeptidase that has been immobilised onto beaded agarose resin to enable generation and purification of Fab and F(ab')2 fragments from antibodies.
j.m.: 1 * 5 mL


Producent: Thermo Fisher Scientific
Opis: Pierce™ Detergent removal resin enables removal of commonly used ionic, non-ionic and zwitterionic detergents from protein/peptide digest solutions.

Producent: Thermo Fisher Scientific
Opis: The HiPPR™ Detergent Removal Spin Column Kit improves mass spectrometry results by efficiently removing detergents from 25 to 200 µl samples with low protein or peptide concentrations.

Numer katalogowy: (PIER20341)
Producent: Thermo Fisher Scientific
Opis: Pierce™ Immobilised Papain consists of a cysteine-endopeptidase that has been immobilised onto beaded agarose resin to enable generation and purification of Fab fragments from antibodies.
j.m.: 1 * 5 mL


Numer katalogowy: (PIER20308)
Producent: Thermo Fisher Scientific
Opis: SDS-Out™ SDS Precipitation Kit provides convenient removal of SDS from small-volume (0,1 to 10 ml) protein samples.
j.m.: 1 * 1 KIT


Numer katalogowy: (PIER88216)
Producent: Thermo Fisher Scientific
Opis: This Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.
j.m.: 1 * 1 SZT


Numer katalogowy: (786-791)
Producent: G-Biosciences
Opis: A proteolytic enzyme immobilised on 4% agarose that is routinely used for the generation of F(ab’)₂ fragments from Immunoglobulin G (IgG). The pepsin has the ability to cleave the heavy chains near the hinge region. The immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.
j.m.: 1 * 1 SZT


Numer katalogowy: (786-790)
Producent: G-Biosciences
Opis: A cysteine protease enzyme (EC 3.4.22.2) immobilised on 4% agarose, cleaves Immunoglobulin G (IgG) antibody molecules in the hinge region, generating three ~50 kDa fragments; two Fab domains and a Fc domain. The papain-digested antibody is unable to promote agglutination, precipitation, opsonisation, and lysis.
j.m.: 1 * 1 SZT


Producent: MP Biomedicals
Opis: Lysing Matrix Z is used primarily for lysis of animal and plant tissue, tougher stems, roots and seeds, and whole insects. Each impact-resistant tube contains yttria-stabilised zirconium oxide beads.
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Zapasy tego artykułu są ograniczone, ale możliwe, że jest on dostępny w pobliskim magazynie. Upewnij się, że jesteś zalogowany na stronie, aby móc sprawdzić dostępność zapasów. Jeśli call ciągle się wyświetla i potrzebujesz pomocy, zadzwoń na 58 323 82 00.
Zapasy tego artykułu są ograniczone, ale możliwe, że jest on dostępny w pobliskim magazynie. Upewnij się, że jesteś zalogowany na stronie, aby móc sprawdzić dostępność zapasów. Jeśli call ciągle się wyświetla i potrzebujesz pomocy, zadzwoń na 58 323 82 00.
Ten produkt jest ograniczony w dostępie i można go zamówić tylko przy użyciu zaaprobowanego konta. Jeśli potrzebujesz pomocy, napisz do Działu Prawnego VWR na tomasz.chrobak@pl.vwr.com
Do zakupu tego produktu może być potrzebna dodatkowa dokumentacja. Przedstawiciel VWR skontaktuje się z Tobą w razie potrzeby.
Ten produkt jest zablokowany na stronie internetowej. W celu zamówienia, skontaktuj się z Działem Obsługi Klienta.
Produkt oryginalny został wycofany. Dostępny jest wskazny zamiennik .
Wybrany produkt został wycofany - sprzedaż do wyczerpania zapasów. Dostępne zamienniki można znaleźć poprzez wpisanie powyższego numeru katalogowego VWR w okno wyszukiwarki. Jeśli potrzebujesz dalszej pomocy, prosimy o kontakt z Działem Doradztwa Produktowego pod numerem telefonu 58 3238 220.
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