Producent: ENZO LIFE SCIENCES

Opis: Concentrated lysis buffer for preparation of cell and tissue extracts.

Producent: Merck Millipore (Novagen)

Opis: The CytoBuster™ protein extraction reagent is a proprietary formulation of detergents optimised for efficient extraction of soluble proteins from mammalian and insect cells. The gentle, non-ionic composition of the reagent enables isolation of functionally active endogenous or expressed proteins without a need for secondary treatment such as sonication or freeze/thaw.

Numer katalogowy: (ENZOBMLKI1050300)

Producent: ENZO LIFE SCIENCES

Opis: FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS® Developer produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1x Developer dilution (e.g. Trichostatin A) for class I and II HDACs, nicotinamide for sirtuins).

j.m.: 1 * 300 µl

Wyprzedaż

Numer katalogowy: (PIER90067)

Producent: Thermo Fisher Scientific

Opis: Pierce™ TMTzero™ Label Reagent serves as a "light" tag for MS-level peptide quantitation similar to duplex isotopic metabolic labeling, such as SILAC, or isotopic dimethylation labeling.

j.m.: 1 * 1 KIT

Wyprzedaż

Producent: G-Biosciences

Opis: Detergents are essential for protein solubility during protein extraction and sample preparation, especially when working with hydrophobic proteins. The presence of high concentrations of detergents in protein samples can impair ELISA, IEF, protease digestion of proteins and suppress peptide ionisation when analysed by mass spectrometry.

Numer katalogowy: (786-241)

Producent: G-Biosciences

Opis: InGel™ provides a reliable method for the proteolytic digestion and subsequent extration of silver stained proteins in gel for subsequent analysis by mass spectrometry.

j.m.: 1 * 1 KIT

Wyprzedaż

Producent: G-Biosciences

Opis: Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to β-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider range of pH, including lower acidic pHs. Protein-S-S-Reductant™ completely reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions. This ready to use solution is at a neutral pH and stabilised for long-term storage. Simply supplement Protein-S-S-Reductant™ in place of DTT or β-mercaptoethanol and boil the sample.

Numer katalogowy: (786-PSB)

Producent: G-Biosciences

Opis: FOCUS™ protein solubilisation buffer is a dry, urea-based buffer for protein solubilisation. It is a pre-mixed formulation of urea, thiourea, CHAPS and non detergent sulphobetaine for maximum solubilising strength.

j.m.: 1 * 50 mL

Wyprzedaż

Producent: G-Biosciences

Opis: DetergentOUT™ Tween® is a spin column format detergent removal resin for polysorbate or Tween® detergents or surfactants.

Certyfikaty

Producent: G-Biosciences

Opis: The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research. A crucial step in using peptides as antigens is their coupling to carrier proteins. Peptides are designed to be good epitopes by themselves; however they fail to elicit a strong antibody response. The reason for this is that the epitopes are able to bind to the B-cells, but are too small to contain additional motifs required to associate with class II molecules and T-cell receptors. The carrier proteins, once coupled to the peptides, provide these additional motifs and generate a strong antibody response. All three carrier proteins are also offered as activated carrier proteins, named as ActiveHOOK™.

Numer katalogowy: (786-599)

Producent: G-Biosciences

Opis: Immobilised Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose. The resin allows for the purification of avidin, streptavidin and Neutravidin and their subsequent gentle elution using non denaturing elution buffers.

j.m.: 1 * 5 mL

Wyprzedaż

Numer katalogowy: (PIER90053)

Producent: Thermo Fisher Scientific

Opis: Asp-N protease is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications.

j.m.: 1 * 2 µG

MSDS Wyprzedaż

Producent: Thermo Fisher Scientific

Opis: Neuronal culture media supplement (50X), sufficient for 500 ml of neuronal culture medium, Pierce

Numer katalogowy: (PIER90054)

Producent: Thermo Fisher Scientific

Opis: Glu-C protease is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications.

j.m.: 1 * 50 µG

MSDS Wyprzedaż

Numer katalogowy: (PIER90056)

Producent: Thermo Fisher Scientific

Opis: Chymotrypsin protease is a purified native endoproteinase validated for maximum activity and selectivity in proteomic applications.

j.m.: 1 * 100 µG

Wyprzedaż

Producent: ENZO LIFE SCIENCES

Opis: Antigen retrieval reagent is used to recover antigens masked by fixation in cross linking fixatives such as formalin.