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Product Image-K1232

PCR cloning kit, CloneJET

Producent: Thermo Fisher Scientific
Opis: The CloneJET PCR cloning kit contains a ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Recircularised pJET1.2/blunt vector molecules lacking an insert express a lethal restriction enzyme, which kills the host <i>E. coli</i> cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection.

Product Image-EL0016

T4 DNA ligase

Numer katalogowy: (EL0016)
Producent: Thermo Fisher Scientific
Opis: This T4 DNA ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.
j.m.: 1 * 1 KIT

Certyfikaty


Product Image-710-0203

Cylindry do klonowania

Numer katalogowy: (710-0203)
Producent: HILGENBERG
Opis: Borosilicate glass 3.3.
j.m.: 1 * 100 SZT
Product Image-27-0361-01

Ligaza T4 DNA, Ready-To-Go™

Numer katalogowy: (27-0361-01)
Producent: Cytiva
Opis: T4 DNA ligase catalyses the formation of a phosphodiester bond between the 5'-phosphoryl group and the 3'-hydroxyl group of two double-stranded DNA fragments. ATP is required for this reaction.
j.m.: 1 * 50 Tests
Product Image-GA1100-10

Zestaw etapowy Gibson Assembly® HiFi 1

Producent: SGI - DNA Synthetic Genomics
Opis: The Gibson Assembly® HiFi 1 Step Kit provides a simple and effective method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method allows researchers to achieve complex assembly of large DNA constructs, with multiple inserts, in 1 hour.

MSDS

Product Image-SYGEGA1100-4XS

Gibson Assembly® HiFi HC Cloning Kits and Master Mixes

Producent: SGI - DNA Synthetic Genomics
Opis: Gibson Assembly® HiFi HC 1-Step Kits and Master Mixes allow restriction digest-free, cloning of one or more DNA fragments into virtually any location of any plasmid vector in a single round of cloning.

Image Unavailable-70573-3

Ligation premix, Clonables™

Producent: Merck Millipore (Novagen)
Opis: Clonables™ 2X ligation premix is a single solution containing optimised concentrations of T4 DNA ligase, buffer, stabiliser, and cofactors needed for efficient ligation of any type of compatible DNA ends.

Product Image-K1261

LIC cloning and expression kits, aLICator™, Fermentas

Producent: Thermo Fisher Scientific
Opis: The aLICator™ LIC Cloning and Expression System is designed for fast and efficient ligation independent cloning and tight regulation of gene expression in <i>E. coli</i>. The plate bacterial expression vectors are designed for high levels of target protein expression in concert with minimal basal (uninduced) expression, which permits expression of proteins that are toxic to <i>E. coli</i> cells.

Product Image-GA1200-50

Zestaw Ultra Gibson Assembly®

Producent: SGI - DNA Synthetic Genomics
Opis: The Gibson Assembly® Ultra Kit provides a robust and efficient method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method is designed for complex assembly of 2 to 15 large DNA constructs using only small amounts (nanograms) of DNA.

MSDS

Product Image-GA1200-10MM

Zestaw Ultra mieszaniny Master Gibson Assembly® Ultra Master Mix

Producent: SGI - DNA Synthetic Genomics
Opis: The Gibson Assembly® Ultra reaction occurs in two steps using the addition of two master mixes in two sequential steps. In the first step, the GA Ultra Master Mix A (2X) creates single-strand DNA 5’ overhangs by chewing back DNA from the 3’ end. This reaction is cooled under conditions that allow for annealing of complementary overlap regions. Next, the Gibson Assembly® Ultra Master Mix B (2X) is added. During this step, nucleotides are incorporated into the construct to fill in the gaps in the annealed DNA fragments and nicks are sealed to create a contiguous DNA construct.

MSDS

Product Image-GA1100-10MM

Zestaw etapowy mieszanin Master Gibson Assembly® HiFi 1

Producent: SGI - DNA Synthetic Genomics
Opis: The Gibson Assembly® Hi-Fi 1-Step Master Mix (2X) contains a proprietary mixture of enzymes and reagents optimised to facilitate one-step assembly of double standed DNA fragments. This master mix includes a proof-reading polymerase that mediates junction repair resulting assembled constructs with low rates of junction errors and high sequence fidelity.

MSDS

Image Unavailable-70014-3

T7Select® phage display system, Novagen®

Producent: Merck Millipore (Novagen)
Opis: Novagen® T7Select® Phage Display System takes advantage of the properties of bacteriophage T7. This system is easy to use and has the capacity to display peptides up to about 50 amino acids in size in high copy number (415 per phage), and peptides or proteins up to about 1200 amino acids in low copy number (0,1 to 1 per phage) or mid-copy number (5 to 15 per phage).

Product Image-391-1987

Cylindry do klonowania, SP Bel-Art

Producent: Bel-Art Products, a Part of SP
Opis: These cloning cylinders facilitate the isolation of individual cell clones, each generated from a single cell, yielding a homogenous population of cells.

Product Image-BRND232408

Cylindry miarowe do mieszania, wysokie, klasa A, szkło borokrzemowe, BLAUBRAND®

Producent: Brand
Opis: Borosilicate glass 3.3, class A, conformity certified.

Product Image-612-5667

VWR®, cylindry miarowe, wysokie, klasa B, PP

Producent: VWR Collection
Opis: PP, przejrzyste, wysoka forma, klasa B.

Product Image-612-5845

VWR®, Cylindry miarowe, Klasa A, TPX

Producent: VWR Collection
Opis: Made from TPX , 100 % virgin resin, clear.

Certyfikaty

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Zapasy tego artykułu są ograniczone, ale możliwe, że jest on dostępny w pobliskim magazynie. Upewnij się, że jesteś zalogowany na stronie, aby móc sprawdzić dostępność zapasów. Jeśli call ciągle się wyświetla i potrzebujesz pomocy, zadzwoń na 58 323 82 00.
Ten produkt jest ograniczony w dostępie i można go zamówić tylko przy użyciu zaaprobowanego konta. Jeśli potrzebujesz pomocy, napisz do Działu Prawnego VWR na tomasz.chrobak@pl.vwr.com
Do zakupu tego produktu może być potrzebna dodatkowa dokumentacja. Przedstawiciel VWR skontaktuje się z Tobą w razie potrzeby.
Ten produkt jest zablokowany na stronie internetowej. W celu zamówienia, skontaktuj się z Działem Obsługi Klienta.
Produkt oryginalny został wycofany. Dostępny jest wskazny zamiennik .
Wybrany produkt został wycofany - sprzedaż do wyczerpania zapasów. Dostępne zamienniki można znaleźć poprzez wpisanie powyższego numeru katalogowego VWR w okno wyszukiwarki. Jeśli potrzebujesz dalszej pomocy, prosimy o kontakt z Działem Doradztwa Produktowego pod numerem telefonu 58 3238 220.
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