Wyszukano: G Biosciences

Producent: G-Biosciences

Opis: The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions, however is dramatically affected by the presence of reducing agents, including DTT, ß-mercaptoethanol and TCEP.

Certyfikaty

Producent: G-Biosciences

Opis: Detergents are essential for protein solubility during protein extraction and sample preparation, especially when working with hydrophobic proteins. The presence of high concentrations of detergents in protein samples can impair ELISA, IEF, protease digestion of proteins and suppress peptide ionisation when analysed by mass spectrometry.

Numer katalogowy: (DG097)

Producent: G-Biosciences

Opis: CHAPS (3-[(3-cholamidopropylo)-dimetyloamonio]-propano-sulfonian) 5% in aqueous solution

j.m.: 1 * 50 mL

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Numer katalogowy: (BE-407)

Producent: G-Biosciences

Opis: This lab activity has two objectives - to deepen the understanding of protein molecules and for students to learn the potential of electrophoresis in protein analysis. Students study the fundamentals of protein structure from their primary structure to the more complex tertiary and quaternary structures, utilising protein electrophoresis. Complex mixtures of protein samples and characterised pure protein samples, in conjunction with electrophoresis, are utilised to study protein structure and the potential of protein electrophoresis. Using non-denaturing and denaturing electrophoresis, students understand the difference between primary, tertiary and quaternary structures, the importance of disulfhydryl bridges in maintaining protein structure and electrophoresis in studying complete proteins and protein subunits.

j.m.: 1 * 1 KIT

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Numer katalogowy: (BE-415)

Producent: G-Biosciences

Opis: Ion exchange chromatography is used to separate charged molecules from complex biological samples. The charged molecules bind to a solid support carrying an opposite charge to the molecule. Proteins contain regions of charged groups on the surface which interact with the ion exchange groups immobilised on the solid support (resin column). Immobilised proteins are eluted by changing either pH or the salt gradient or a combination of both. This lab activity involves preparation of a crude protein extract and running ion exchange chromatography for isolation of proteins.

j.m.: 1 * 1 KIT

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Numer katalogowy: (BE-408)

Producent: G-Biosciences

Opis: In this lab activity, students learn to perform simple protein isolation procedures to isolate the protein fingerprint from various fresh tissues. They will compare the protein fingerprints of four different tissues to understand that the function of a particular organ is due to the proteins that are localised to the specific organ. Also included in this kit are four dried protein samples (from mouse liver, brain, heart and lungs) to compare as a control, if fresh tissues cannot be obtained.

j.m.: 1 * 1 KIT

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Producent: G-Biosciences

Opis: T4 UvsX DNA recombinase mediates DNA strand exchange between homologous chromosomes. The protein forms a right-handed nucleoprotein complex on single ssDNA called the presynaptic filament that can search for homology in duplex DNA and pair the recombining DNA molecules to form a DNA joint molecule. This process is aided by ssDNA binding proteins and recombination mediators. The filament is then elongated by DNA polymerase with the newly synthesised strand displacing the old strand. The newly synthesised duplex DNA can then act as a template for the next cycle to achieve exponential amplification of dsDNA. <i>E.coli</i> cells expressing recombinant T4 UvsX gene.

Producent: G-Biosciences

Opis: wodorowęglan sodu

Producent: G-Biosciences

Opis: Mocznik, Ultra Pure Grade

Producent: G-Biosciences

Opis: Immobilised Protein A and G are designed for binding the constant domains of immunoglobulin (Ig) molecules. Immobilised Protein A is coupled to 6% highly cross-linked agarose beads by a proprietary coupling method that provides high coupling efficiency for immunoglobulins and minimal protein A leaching. The binding capacity of Protein A is 18 to 43 mg Human IgG per ml resin.

Producent: G-Biosciences

Opis: RUBEO™ is a fluorescent protein stain that is ideal for staining proteins in 1D and 2D electrophoresis gels.

Producent: G-Biosciences

Opis: TCM-ProteaseArrest™ is a mixture of protease inhibitors with a broad specificity for the inhibition of serine, cysteine, aspartic (acid) proteases and aminopeptidases.

Numer katalogowy: (GENO786-1999)

Producent: G-Biosciences

Opis: Bsu DNA Polymerase. Large fragment is from Bacillus subtilis and is modified to retain its 5'→ 3' DNA polymerase activity with its 5'→ 3' exonuclease domain removed. It also lacks the 3'→ 5' exonuclease activity.

j.m.: 1 * 1 KIT

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Numer katalogowy: (GENO786-1992)

Producent: G-Biosciences

Opis: T4 recombination mediator protein, UvsY plays important roles in homologous recombination (HR). During HR, the UvsX recombinase competes with the prebound single-stranded binding protein gp32 for DNA-binding sites and UvsY stimulates this filament nucleation event.

j.m.: 1 * 100 µG

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Producent: G-Biosciences

Opis: Protein binding membrane strips

Numer katalogowy: (M006)

Producent: G-Biosciences

Opis: siarczan (VI) magnezu

j.m.: 1 * 100 g

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