Wyszukano: Biowest

Producent: Biowest

Opis: RPMI are general purpose enriched media with extensive applications for a broad spectrum of mammalian and hybridoma cells, including human myeloma, mouse hybridoma, human leukocytes, and B and T lymphocytes. It was originally formulated for suspension cultures and monolayer culture of human leukaemia cells.

Certyfikaty

Producent: Biowest

Opis: Studies to determine the nutritional requirements of many cells have been in progress since Eagle's first reports. The major essential nutrients were identified, and work became focussed on the media requirements of individual cell types. Many media designed for these purposes are now available. Among the first of these media, developed initially to study hormonal requirements of cells in culture, was a mixture of DMEM and Ham's F12 medium, known as DMEM-Ham's F12.

MSDS

Producent: Biowest

Opis: Antibiotic G418 is an aminoglycoside antibiotic similar in structure to gentamycin. It exhibits toxicity towards both eukaryotic and prokaryotic cells. The optimal concentration for selection and maintenance must be determined for each cell line.

MSDS Certyfikaty

Producent: Biowest

Opis: Minimum Essential Medium (MEM) with Earle's Balanced Salts is a modification of Eagle's earlier medium, Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. These media have demonstrated the ability to support a variety of normal and transformed cells in culture and contain Earle's Balanced Salts, which make them suitable for use in atmospheres charged with CO₂ gas.

MSDS Certyfikaty

Producent: Biowest

Opis: Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge the cells from their substrate depends on the sensitivity of the cells.

MSDS Certyfikaty

Producent: Biowest

Opis: Ham's F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. It is frequently used with dialysed serum, hormones, selenium and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat and has proved to be an excellent cloning medium for the culture of myeloma and hybrid cells.

MSDS Certyfikaty

Numer katalogowy: (L0014-100)

Producent: Biowest

Opis: Glutamine penicillin streptamycin acts against gram-negative and gram-positive bacteria.

j.m.: 1 * 100 mL

Certyfikaty Wyprzedaż

Numer katalogowy: (L0207-500)

Producent: Biowest

Opis: Schneider’s Insect Medium was developed to support the growth of excised imaginal discs from the fruit fly, <i>Drosophila melanogaster</i>. When supplemented with 5 to 20% heat-inactivated foetal bovine serum Schneider's mediuam has been found to support the rapid growth of both primary and established cultures of cells derived from Drosophila spp. and several other dipterans.

j.m.: 1 * 500 mL

MSDS Certyfikaty Wyprzedaż

Numer katalogowy: (L0950-100)

Producent: Biowest

Opis: Accustase is a ready to use cell detachment solution of proteolytic and collagenolytic enzymes. Accutase is a direct gentle replacement for trypsin solution. It can also be used on suspension cells to reduce their agglutination in order to ease counting.

j.m.: 1 * 100 mL

MSDS Certyfikaty Wyprzedaż

Producent: Biowest

Opis: Colcemid arrests the division of cells in mitosis. It prevents the formation of the spindle apparatus responsible for cell division, thereby permitting an accumulation of metaphases.

MSDS Certyfikaty

Producent: Biowest

Opis: Amphotericin B used in tissue culture to prevent growth of yeasts and fungi. It changes the function and integrity of eucaryotic cell membranes by forming complexes with sterols (cholesterol) hence having no effect on bacteria. Cause leakage of glucose.

MSDS Certyfikaty

Numer katalogowy: (X0550-100)

Producent: Biowest

Opis: L(+)-glutamina 0.2 mol/l roztwór wodny

j.m.: 1 * 100 mL

MSDS Certyfikaty Wyprzedaż

Producent: Biowest

Opis: Gentamicin sulfate acts against gram-negative, gram-positive bacteria and mycoplasma.

MSDS Certyfikaty

Producent: Biowest

Opis: The Leibovitz L-15 media were formulated to promote the cell growth in medium not balanced in CO₂. The formulations were developed with the sodium bicarbonate buffer. The Leibovitz L-15 media are buffered by a complement of salts, free base amino acids and galactose so they can be used under conditions of free gaseous exchange with the atmosphere. When properly supplemented, L-15 Medium supports established cell lines, such as HEp-2 and LLC-MK2, as well as primary explants of embryonic and adult human tissue. Many viruses also have been successfully cultivated in this medium.

MSDS Certyfikaty

Producent: Biowest

Opis: Ham's F10 medium is intended to support the growth of Chinese Hamster Ovary cells under serum-free conditions and other mammalian cell types with serum supplementation. It is a popular medium for growth of fastidious cell lines.

Certyfikaty

Producent: Biowest

Opis: L-glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. It is a crucial component of many cell culture media and serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. However, in liquid media or stock solutions, L-glutamine can degrade relatively rapidly. L-glutamine is also more labile in cell culture solution than other amino acids.

MSDS Certyfikaty