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Product Image-SOLUL-7606-5

TriLink® CleanCap® Cas9 mRNA

Producent: TriLink BioTechnologies
Opis: Cas9 mRNA expresses a version of the <em>Streptococcus pyogenes</em> SF370 Cas9 protein (CRISPR Associated Protein 9). Cas9 functions as part of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) genome editing system. In the CRISPR system, an RNA guide sequence targets the site of interest and the Cas9 protein is employed to perform double stranded DNA cleavage.

Image Unavailable-SOLUL-7802-1000

CleanCap® AU FLuc saRNA

Producent: TriLink BioTechnologies
Opis: <p><span style="color:black">This construct expresses a firefly luciferase (FLuc) protein, originally isolated from the firefly <span style="color:black"><em>Photinus pyralis</em></span><span style="color:black">, as self-amplifying RNA (saRNA). FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.</span></span></p>

New Product

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CytoLiner™ Fixed Cell Membrane Stains

Producent: Biotium
Opis: CytoLiner™ Fixed cell membrane dyes are novel lipophilic fluorescent dyes developed specifically for selective plasma membrane staining in fixed cells for microscopy. The dyes are engineered for robust and consistent staining of formaldehyde-fixed cells, and are suitable for downstream immunofluorescence staining protocols.

New Product

Image Unavailable-SOLUL-7801-100

CleanCap® AU EGFP saRNA

Producent: TriLink BioTechnologies
Opis: <p><span style="color:black">This construct expresses an enhanced version of the green fluorescent protein (EGFP), originally isolated from the jellyfish <span style="color:black"><em>Aequorea victoria</em></span><span style="color:black">, as self-amplifying RNA (saRNA). EGFP is a commonly used direct detection reporter in mammalian cells, yielding bright green fluorescence with an emission peak at 509 nm.</span></span></p>

New Product

Product Image-SOLUL-8106-5

TriLink® CleanCap® M6 Cas9 mRNA (N1MePsU)

Producent: TriLink BioTechnologies
Opis: <p>Cas9 mRNA expresses a version of the <em>Streptococcus pyogenes </em>SF370 Cas9 protein (CRISPR Associated Protein 9). Cas9 functions as part of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) genome editing system. In the CRISPR system, an RNA guide sequence targets the site of interest and the Cas9 protein is employed to perform double-stranded DNA cleavage.</p>

New Product

Product Image-K1232

PCR cloning kit, CloneJET

Producent: Thermo Fisher Scientific
Opis: The CloneJET PCR cloning kit contains a ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Recircularised pJET1.2/blunt vector molecules lacking an insert express a lethal restriction enzyme, which kills the host <i>E. coli</i> cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection.

Product Image-23005.

Fluorescencyjne antyblaknięciowe media EverBrite™ i uszczelniająca pokrywka CoverGrip™

Producent: Biotium
Opis: EverBrite™ mounting media are anti-fade mountants for preserving fluorescence during microscopy. EverBrite™ mounting media prevent rapid photobleaching of fluorescein, rhodamine, and cyanine based dyes and are optimally formulated for use with Biotum’s CF™ dye conjugates. Unlike Vectashield® EverBrite™ mounting media is compatible with Cy® dyes such as Cy®2, Cy®3 and Cy®5. EverBrite™ mounting medium is a wet-set medium while EverBrite™ hardset mounting medium cures overnight to form a hard permanent seal with the coverslip, eliminating the need to seal coverslip edges with nail polish or other sealants. Both formulations are available with DAPI for blue fluorescent nuclear counterstaining without an additional staining step.

MSDS

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Proteomic Protein Controls

Producent: G-Biosciences
Opis: Protein preparations specifically prepared for standardising electrophoresis methods and protocols. Substantially free from non-protein agents such as nucleic acids, detergents, salts, lipids and other common laboratory agents, the protein preparation has low conductivity (<50 μs). Supplied as dry protein pellets, simply re-hydrate and use. Proteomic protein sample controls are available for animal cells, <i>E.coli</i>, yeast, plant or as a control set that contains one 2 mg vial of each.

Product Image-786-077

Redukcja czynników ba?kowych

Producent: G-Biosciences
Opis: Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to β-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider range of pH, including lower acidic pHs. Protein-S-S-Reductant™ completely reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions. This ready to use solution is at a neutral pH and stabilised for long-term storage. Simply supplement Protein-S-S-Reductant™ in place of DTT or β-mercaptoethanol and boil the sample.

Image Unavailable-ENZOBMLKI1761250

Koncentrat Fluor-de-Lys® Developer II

Numer katalogowy: (ENZOBMLKI1761250)
Producent: ENZO LIFE SCIENCES
Opis: FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates. Deacetylation of the FLUOR DE LYS® substrate sensitises it so that, in a second step, treatment with the FLUOR DE LYS® Developer II produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1X Developer dilution (e.g. nicotinamide for sirtuins).
j.m.: 1 * 1,25 mL
Image Unavailable-SOLUK-7413-25

CleanCap® AG (3′ OMe) CleanScript™ IVT Kit

Numer katalogowy: (SOLUK-7413-25)
Producent: TriLink BioTechnologies
Opis: <p><span style="color:black">TriLink’s CleanCap® AG (3′ OMe) CleanScript™ IVT kit provides essential components for mRNA synthesis using co-transcriptional capping by in vitro transcription (IVT). The kit components are sufficient to run 25 of 100 µl reactions following our recommended IVT protocol with co-transcriptional capping. It includes a cap analog, modified and unmodified nucleotides, IVT enzymes including CleanScribe RNA polymerase, 10X CleanScript IVT reaction buffer, and a linearized FLuc DNA control template.</span></p>
j.m.: 1 * 100 µl

New Product


Image Unavailable-OZBIIV-BF30500

Odczynnik do transfekcji, BrainFectIN

Producent: OZ BIOSCIENCES
Opis: BrainFectIN transfection reagent enables nucleic acid delivery into the central nervous system of small animals.

Image Unavailable-580561-1

TCEP, neutral, Calbiochem®

Numer katalogowy: (580561-1)
Producent: Merck Millipore (Calbiochem‎)
Opis: A ready-to-use stable, neutral form of the disulfide-reducing agent TCEP, hydrochloride.
j.m.: 1 * 1 mL
Product Image-786-586

Rekombinowana streptawidyna

Producent: G-Biosciences
Opis: Recombinant streptavidin from S<i>treptomyces avidinii</i>, produced in <i>E.coli.</i>

Image Unavailable-ENZOBMLKI1050300

Koncentrat Fluor-de-Lys® Developer I

Numer katalogowy: (ENZOBMLKI1050300)
Producent: ENZO LIFE SCIENCES
Opis: FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS® Developer produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1x Developer dilution (e.g. Trichostatin A) for class I and II HDACs, nicotinamide for sirtuins).
j.m.: 1 * 300 µl
Image Unavailable-ICNA08642851

Kompletny adiuwant Freunda

Numer katalogowy: (ICNA08642851)
Producent: MP Biomedicals
Opis: Freund's complete adjuvant is made by a modification of Freund's technique, in which 25 mg of <i>Mycobacterium</i> is suspended in a mixture of 7,5 ml Arlacel A and 42,5 ml paraffin oil.
j.m.: 1 * 50 mL
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Zapasy tego artykułu są ograniczone, ale możliwe, że jest on dostępny w pobliskim magazynie. Upewnij się, że jesteś zalogowany na stronie, aby móc sprawdzić dostępność zapasów. Jeśli call ciągle się wyświetla i potrzebujesz pomocy, zadzwoń na 58 323 82 00.
Ten produkt jest ograniczony w dostępie i można go zamówić tylko przy użyciu zaaprobowanego konta. Jeśli potrzebujesz pomocy, napisz do Działu Prawnego VWR na tomasz.chrobak@pl.vwr.com
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Produkt oryginalny został wycofany. Dostępny jest wskazny zamiennik .
Wybrany produkt został wycofany - sprzedaż do wyczerpania zapasów. Dostępne zamienniki można znaleźć poprzez wpisanie powyższego numeru katalogowego VWR w okno wyszukiwarki. Jeśli potrzebujesz dalszej pomocy, prosimy o kontakt z Działem Doradztwa Produktowego pod numerem telefonu 58 3238 220.
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