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Wyszukano: 3-(3-Bromophenyl)prop-2-yn-1-ol


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Opis: ortofosforan (V) sodu dodekahydrat 98.0-102.0%, kryształy, AR® ACS, Macron Fine Chemicals™
Numer katalogowy: 7940-20
j.m.: 1 * 12 kg
Producent: Avantor


Opis: diwodorofosforan sodu dihydrat for HPLC
Numer katalogowy: ACRO446222500
j.m.: 1 * 250 g
Producent: Thermo Fisher Scientific

Certyfikaty


Opis: Crystals. Lot analysis on label.
Numer katalogowy: 3836-07
j.m.: 1 * 12 kg
Producent: Avantor

Certyfikaty


Opis: ortofosforan (V) sodu dodekahydrat ≥97%
Numer katalogowy: A15713.0E
j.m.: 1 * 2.500 g
Producent: Thermo Fisher Scientific

MSDS Certyfikaty


Opis: Phosphate, NANOCONTROL®, single standards, 1,00 mg/l PO₄-P
3,07 mg/l PO₄3-
Numer katalogowy: MANA92576
j.m.: 1 * 1 KIT
Producent: MACHEREY-NAGEL

MSDS


Opis: diwodorofosforan sodu dihydrat 98%, Extra Pure
Numer katalogowy: ACRO447760050
j.m.: 1 * 5 kg
Producent: Thermo Fisher Scientific

MSDS


Opis: Pokemon, the POK erythroid myeloid ontogenic factor, not only regulates the expression of many genes, but also plays an important role in cell tumorigenesis. To investigate the molecular mechanism regulating expression of the Pokemon gene in humans, its 5'-upstream region was cloned and analyzed. Transient analysis revealed that the Pokemon promoter is constitutive. Deletion analysis and a DNA decoy assay indicated that the NEG-U and NEG-D elements were involved in negative regulation of the Pokemon promoter, whereas the POS-D element was mainly responsible for its strong activity. Electrophoretic mobility shift assays suggested that the NEG-U, NEG-D and POS-D elements were specifically bound by the nuclear extract from A549 cells in vitro. Mutation analysis demonstrated that cooperation of the NEG-U and NEG-D elements led to negative regulation of the Pokemon promoter. Moreover, the NEG-U and NEG-D elements needed to be an appropriate distance apart in the Pokemon promoter in order to cooperate. Taken together, our results elucidate the mechanism underlying the regulation of Pokemon gene transcription, and also define a novel regulatory sequence that may be used to decrease expression of the Pokemon gene in cancer gene therapy.
Numer katalogowy: BOSSBS-10235R
j.m.: 1 * 100 µl
Producent: Bioss


Opis: Pokemon, the POK erythroid myeloid ontogenic factor, not only regulates the expression of many genes, but also plays an important role in cell tumorigenesis. To investigate the molecular mechanism regulating expression of the Pokemon gene in humans, its 5'-upstream region was cloned and analyzed. Transient analysis revealed that the Pokemon promoter is constitutive. Deletion analysis and a DNA decoy assay indicated that the NEG-U and NEG-D elements were involved in negative regulation of the Pokemon promoter, whereas the POS-D element was mainly responsible for its strong activity. Electrophoretic mobility shift assays suggested that the NEG-U, NEG-D and POS-D elements were specifically bound by the nuclear extract from A549 cells in vitro. Mutation analysis demonstrated that cooperation of the NEG-U and NEG-D elements led to negative regulation of the Pokemon promoter. Moreover, the NEG-U and NEG-D elements needed to be an appropriate distance apart in the Pokemon promoter in order to cooperate. Taken together, our results elucidate the mechanism underlying the regulation of Pokemon gene transcription, and also define a novel regulatory sequence that may be used to decrease expression of the Pokemon gene in cancer gene therapy.
Numer katalogowy: BOSSBS-10235R-A647
j.m.: 1 * 100 µl
Producent: Bioss


Opis: Pokemon, the POK erythroid myeloid ontogenic factor, not only regulates the expression of many genes, but also plays an important role in cell tumorigenesis. To investigate the molecular mechanism regulating expression of the Pokemon gene in humans, its 5'-upstream region was cloned and analyzed. Transient analysis revealed that the Pokemon promoter is constitutive. Deletion analysis and a DNA decoy assay indicated that the NEG-U and NEG-D elements were involved in negative regulation of the Pokemon promoter, whereas the POS-D element was mainly responsible for its strong activity. Electrophoretic mobility shift assays suggested that the NEG-U, NEG-D and POS-D elements were specifically bound by the nuclear extract from A549 cells in vitro. Mutation analysis demonstrated that cooperation of the NEG-U and NEG-D elements led to negative regulation of the Pokemon promoter. Moreover, the NEG-U and NEG-D elements needed to be an appropriate distance apart in the Pokemon promoter in order to cooperate. Taken together, our results elucidate the mechanism underlying the regulation of Pokemon gene transcription, and also define a novel regulatory sequence that may be used to decrease expression of the Pokemon gene in cancer gene therapy.
Numer katalogowy: BOSSBS-10235R-CY7
j.m.: 1 * 100 µl
Producent: Bioss


Opis: Pokemon, the POK erythroid myeloid ontogenic factor, not only regulates the expression of many genes, but also plays an important role in cell tumorigenesis. To investigate the molecular mechanism regulating expression of the Pokemon gene in humans, its 5'-upstream region was cloned and analyzed. Transient analysis revealed that the Pokemon promoter is constitutive. Deletion analysis and a DNA decoy assay indicated that the NEG-U and NEG-D elements were involved in negative regulation of the Pokemon promoter, whereas the POS-D element was mainly responsible for its strong activity. Electrophoretic mobility shift assays suggested that the NEG-U, NEG-D and POS-D elements were specifically bound by the nuclear extract from A549 cells in vitro. Mutation analysis demonstrated that cooperation of the NEG-U and NEG-D elements led to negative regulation of the Pokemon promoter. Moreover, the NEG-U and NEG-D elements needed to be an appropriate distance apart in the Pokemon promoter in order to cooperate. Taken together, our results elucidate the mechanism underlying the regulation of Pokemon gene transcription, and also define a novel regulatory sequence that may be used to decrease expression of the Pokemon gene in cancer gene therapy.
Numer katalogowy: BOSSBS-10235R-A350
j.m.: 1 * 100 µl
Producent: Bioss


Opis: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA. Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.
Numer katalogowy: BOSSBS-11304R-CY5
j.m.: 1 * 100 µl
Producent: Bioss


Opis: diwodorofosforan sodu hydrat ≥99.998% (metals basis), Puratronic®
Numer katalogowy: 10870.14
j.m.: 1 * 25 g
Producent: Thermo Fisher Scientific

MSDS Certyfikaty


Opis: diwodorofosforan sodu hydrat 98.0-102.0% ACS
Numer katalogowy: 11591.30
j.m.: 1 * 250 g
Producent: Thermo Fisher Scientific

MSDS Certyfikaty


Opis: Kwas ortofosforowy(V), Sigma-Aldrich®
Numer katalogowy: SIAL466123-25G
j.m.: 1 * 25 g
Producent: Merck


Opis: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA. Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.
Numer katalogowy: BOSSBS-11304R
j.m.: 1 * 100 µl
Producent: Bioss


Opis: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA. Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.
Numer katalogowy: BOSSBS-11304R-CY7
j.m.: 1 * 100 µl
Producent: Bioss


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