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Ovary Lysate

Dostawca: ProSci
PRSI1316-0.1EA 803 PLN
PRSI1316-0.1
Ovary Lysate
Komórki
Ovary tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH 7,9, 1,5 mM MgCl₂, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors).

Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1×SDS sample buffer (50 mM Tris-HCl pH 6,8, 12,5% glycerol, 1% sodium dodecylsulfate, 0,01% bromophenol blue) containing 5% β-mercaptoethanol.
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