Drukuj

VWR®, Fast HiFi DNA Polymerase, 2 U/µl

Dostawca: VWR Chemicals

733-2880EA 630 PLN
733-2880 733-2881 733-2882 733-2883
VWR®, Fast HiFi DNA Polymerase, 2 U/µl
Odczynniki do kwasów nukleinowych Zestawy do end-point PCR

Fast HiFi DNA Polymerase 2 U/µl is a proof reading DNA polymerase displaying the following features; high fidelity >60X Taq DNA Polymerase, ability to amplify problematic DNA targets, such as those with low to high GC content and ability to perform amplification on long DNA targets. lt is recommended for applications, which require extremely high fidelity such as cloning/subcloning, NGS applications and mutagenesis.


  • High fidelity: >60X Taq Fidelity
  • 3’→5’ proof reading exonuclease activity
  • Processes up to 18 kb from complex templates (e.g. human gDNA)
  • Good coverage on DNA templates with low to high GC content
  • Fast extension rate: 10 s/kb


Fast HiFi DNA Polymerase 2 U/µl exhibits both 5'→3' DNA polymerase activity and 3'→5' proof reading exonuclease activity enabling this polymerase to correct base pair mismatches. It is supported with 5X Fast HiFi Buffer allowing robust amplification on DNA targets with low to high GC content and long DNA targets.

For difficult amplicons, such as GC-rich DNA samples, those with complex secondary structures or long amplicons, the addition of 1 to 2 M Betaine enhancer solution is recommended.

For more convenient handling Fast HiFi DNA Polymerase 2 U/µl is also available as a 2X master mix.

Sample types: gDNA, plasmid DNA, bacterial DNA, λDNA and other DNA templates


Uwaga: For Research Use Only. Not for use in diagnostic procedures.

Zobacz ceny

SPECYFIKACJE

Opis Fast HiFi DNA Polymerase 2 U/µl 100 units Fast HiFi DNA Polymerase 2 U/µl 500 units Fast HiFi DNA Polymerase 2 U/µl 1000 units Fast HiFi DNA Polymerase 2 U/µl 2500 units
Environmentally Preferable
Format Enzyme with/without buffer Enzyme with/without buffer Enzyme with/without buffer Enzyme with/without buffer
Cechy feature-ls-33964141|feature-ls-33964143 feature-ls-33964141|feature-ls-33964143 feature-ls-33964141|feature-ls-33964143 feature-ls-33964141|feature-ls-33964143
Gorący start No No No No
Stężenie 2 U/µl 2 U/µl 2 U/µl 2 U/µl
Jednostki 100 500 1000 2500
Rozmiar 1×0,05 ml + 2×1,5 ml 1×0,25 ml + 3×1,5 ml 2×0,25 ml + 6×1,5 ml 5×0,25 ml + 14×1,5 ml
Skład buforu 5X buffer with 7,5 mM MgCl₂ 5X buffer with 7,5 mM MgCl₂ 5X buffer with 7,5 mM MgCl₂ 5X buffer with 7,5 mM MgCl₂
Wysoka wierność Tak Tak Tak Tak
Rozmiar amplikonu 18 kb for gDNA 18 kb for gDNA 18 kb for gDNA 18 kb for gDNA
Processivity Fast Fast Fast Fast
Aktywność egzonukleazy 5’→3’ 3’→5’ 5’→3’ 3’→5’ 5’→3’ 3’→5’ 5’→3’ 3’→5’
Product overhang No No No No
Warunki przechowywania –20 °C (long-term), +4 °C (6 months) –20 °C (long-term), +4 °C (6 months) –20 °C (long-term), +4 °C (6 months) –20 °C (long-term), +4 °C (6 months)
Aplikacja Cloning (high fidelity),Standard amplification Cloning (high fidelity),Standard amplification Cloning (high fidelity),Standard amplification Cloning (high fidelity),Standard amplification

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Więcej About VWR