Acetylocholinoesteraza
Dostawca: Merck
Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.
- Extinction coefficient: E1% = 18,0 (280 nm)
- Isoelectric point: 5,5
- Composition: protein, ≥60% biuret
The enzyme from sigma has been used as a reference to to evaluate the effect of aspartame metabolites on hippocampal acetylcholinesterase activity. The enzyme has also been used in immobilization studies for the rapid detection of acetylthiocholine chloride.
Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine and water to choline and acetic acid.
One unit will hydrolyse 1,0 μmole of acetylcholine to choline and acetate per min at pH 8,0 at 37 °C.
Uwaga: For dilute solutions (<1 mg/ml), add 1 mg/ml BSA to stabilise. Solutions containing at least 1 mg protein/ml in dilute neutral phosphate buffer are stable at least 6 months refrigerated.
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