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VWR® ExoCleanUp FAST, One-step PCR Clean-up

Dostawca: VWR Chemicals

733-2594EA 17800 PLN
733-2594 733-2593 733-2592 733-2849
VWR® ExoCleanUp FAST, One-step PCR Clean-up
Odczynniki do kwasów nukleinowych Odczynniki i zestawy do oczyszczania kwasów nukleinowych

VWR® ExoCleanUp FAST PCR reagent is a one-step PCR clean-up reagent for optimal sequencing results, consisting of a balanced combination of a heat-labile exonuclease I (HL-ExoI) and a recombinant shrimp alkaline phosphatase (rSAP).


  • Designed to clean-up PCR products in 5 minutes
  • No need for spin columns or magnetic beads
  • Treatment improves downstream applications, such as DNA sequencing and SNP analysis


Treatment of amplified PCR products with this reagent helps to remove residual primers and single-stranded DNA, and inactivates excess dNTPs by dephosphorylation. After enzymatic treatment at 37 °C for a minimum of 2 minutes, this reagent is completely inactivated by heating at 80 °C for a minimum of 3 minutes.

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SPECYFIKACJE

Opis ExoCleanUp FAST PCR clean-up reagent ExoCleanUp FAST PCR clean-up reagent ExoCleanUp FAST PCR clean-up reagent ExoCleanUp FAST PCR clean-up reagent
Environmentally Preferable
Aplikacja
Target molecule DNA DNA DNA DNA
Refine target molecule Amplified/modified DNA Amplified/modified DNA Amplified/modified DNA Amplified/modified DNA
Sample type PCR and other enzymatic reactions PCR and other enzymatic reactions PCR and other enzymatic reactions PCR and other enzymatic reactions
Method / Format Enzymatic clean-up Enzymatic clean-up Enzymatic clean-up Enzymatic clean-up
Prep size Scalable Scalable Scalable Scalable
Wielkość próbki 10 × 1 ml 1 × 1 ml 1 × 0,2 ml 4 × 1 ml

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