Producent: FINNZYMES REAGENTS

Opis: Phusion® U hot start PCR master mix is a novel engineered high fidelity enzyme developed using fusion technology.

Producent: FINNZYMES REAGENTS

Opis: Phusion® U green multiplex PCR master mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.

Producent: Thermo Fisher Scientific

Opis: DreamTaq™ Green DNA Polymerase is a combination of DreamTaq™ DNA Polymerase and 10X DreamTaq™ Green Buffer. DreamTaq™ DNA Polymerase is an enhanced Taq DNA polymerase optimised for high throughput PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase.
DreamTaq™ Green DNA Polymerase incorporates modified nucleotides, but is inhibited by dUTP. The 10X DreamTaq™ Green Buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with PCR performance and is compatible with downstream applications such as DNA sequencing,
phosphorylation, ligation and restriction digestion. For applications that require PCR product analysis by absorbance or fluorescence excitation, it is recommended either to use colourless 10X DreamTaq™ Buffer or purify the PCR product prior to analysis.

Producent: FINNZYMES REAGENTS

Opis: Phusion® High-Fidelity PCR Kit offers extreme performance for all major PCR applications. Incorporating Phusion® DNA polymerase, the Phusion® High-Fidelity PCR Kit contains all the reagents necessary, including lambda DNA control template and primers for 1,3 kb and 10 kb amplicons. The template amount is sufficient for performing 20 control reactions in 50 μl volume or 50 control reactions in 20 μl volume.

Producent: FINNZYMES REAGENTS

Opis: Phusion® Flash High-Fidelity PCR Master Mix is a 2X master mix enabling the use of extremely short PCR protocols without compromising either the fidelity or the yield in the reaction. The master mix utilises Phusion® Flash II DNA Polymerase, a proofreading DNA polymerase modified from Phusion® Hot Start II High-Fidelity DNA Polymerase. The fidelity of the Phusion® Flash II DNA Polymerase is 25-fold greater than <i>Taq</i>.

Producent: MP Biomedicals

Opis: Taq-&LOAD™ PCR Master Mix is an optimised 5X reaction mix for PCR containing recombinant <i>Taq</i> DNA polymerase, dNTPs, MgCl₂, and incubation and loading buffers. Also included are a compound increasing the density of the sample and a red/purple dye.

Producent: MP Biomedicals

Opis: <i>Taq</i> DNA Polymerase is a specialised thermostable enzyme isolated from the thermophilic bacterium <i>Thermus aquaticus</i>. The recombinant form of this enzyme is expressed in <i>E. coli</i>. It is highly processive, recombinant polymerase which catalyses the addition of mononucleotide units to the 3’-OH end of a primer chain. It remains functional even after prolonged incubation at 95 °C. This enzyme possesses 5’-3’ exonuclease activiy.

Producent: MP Biomedicals

Opis: The Taq CORE kits are designed for the amplification of specific regions of DNA using PCR. The PCR CORE Kits - Direct Loading provide <i>Taq</i> DNA Polymerase, PCR Direct Loading buffers and dNTPs necessary for amplification and subsequent loading of samples on an agarose gel.

Numer katalogowy: (71975-3)

Producent: Merck Millipore (Novagen)

Opis: KOD Xtreme™ Hot Start DNA Polymerase is an ultra high fidelity DNA polymerase designed for the most challenging PCR applications, including crude sample PCR, amplification of long strand and highly GC-rich DNA templates.

j.m.: 1 * 200 JD

Wyprzedaż

Numer katalogowy: (71007-3)

Producent: Merck Millipore (Novagen)

Opis: The NovaTaq PCR Master Mix is a ready to use 2X concentrated mixture of NovaTaq DNA polymerase, ultra-pure deoxynucleotides, and reaction buffer without magnesium chloride. The master mix simplifies the assembly of PCR reactions and offers advantages of times savings, consistency, and minimal risk of contamination. Simply add the NovaTaq PCR master mix to an equal volume containing the required amount of magnesium chloride, DNA template, and primers, and the reaction is ready for thermal cycling. The final diluted reaction contains 2,5 units of NovaTaq DNA polymerase per 100 µl. Sufficient components are included for 200 standard 50 µl (or 100×100 µl) amplification reactions.

j.m.: 1 * 250 JE

Wyprzedaż

Producent: Merck Millipore (Novagen)

Opis: KOD HiFi DNA Polymerase is a unique proofreading enzyme, isolated from the extreme thermophile <i>Thermococcus kodakaraensis</i> KOD1, which possesses superior processivity and fidelity that enables faster and more accurate PCR amplification than can be achieved with conventional enzymes.

Producent: G-Biosciences

Opis: <i>Pfu</i> DNA Polymerase, derived from the hyperthermophilic archae <i>Pyrococcus furiosus</i>, has superior thermostability and proofreading properties compared to other thermostable polymerases. It has a molecular weight of 90 kDa and can amplify DNA targets up to 2 kb. The elongation velocity is 0,2~0,4 kb/min (70~75 °C). <i>Pfu</i> DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide misincorporation errors. This means that<i> Pfu</i> DNA Polymerase-generated PCR fragments will have fewer errors than <i>Taq</i>-generated PCR inserts. Using <i>Pfu</i> DNA Polymerase results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. <i>Pfu</i> DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis.

Producent: G-Biosciences

Opis: <i>Taq</i> DNA Polymerase is a highly thermostable recombinant DNA polymerase derived from the thermophile <i>Thermus aquaticus</i>. The molecular weight of the recombinant protein is 94 kD. The <i>Taq</i> Polymerase is able to amplify DNA up to 5 kb with an elongation velocity of 0,9 to 1,2 kb/min at 70 to 75 °C. The error rate of this <i>Taq</i> Polymerase is ~2,2×10⁻⁵ nucleotide⁻¹ cycle⁻¹. <i>Taq</i> DNA polymerase catalyses the 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity, and possesses low 5’→3’ exonuclease activity, which results in a 3’-dA overhang on the PCR product

Numer katalogowy: (ICNA11EPTAG100)

Producent: MP Biomedicals

Opis: Taq-&GO™ is a ready to use optimised reaction mix for PCR.

j.m.: 1 * 1 mL

Wyprzedaż

Numer katalogowy: (25-1500-01)

Producent: Cytiva

Opis: illustra™ Hot Start Master Mix is a 2X premixed formulation that can effectively reduce non specific priming and primer-dimer formation during PCR. illustra™ Hot Start Master Mix uses a novel hot start method called primer sequestration.

j.m.: 1 * 1 KIT

Wyprzedaż

Producent: Cytiva

Opis: For PCR and other procedures requiring DNA polymerase activity at elevated temperatures.