Wyszukano: Systemy oczyszczania białek

Producent: Avantor

Opis: The J.T.Baker^® BAKERBOND^® PROchievA™ protein A resin offers superior dynamic binding capacity with excellent alkaline stability linked to Avantor's proprietary ligand.

MSDS Certyfikaty

Producent: Avantor

Opis: Płytki J.T.Baker® Bakerbond® do izolowania białek umożliwiają szybkie usuwanie białek z próbek biologicznych.

Producent: Avantor

Opis: Płytki J.T.Baker® BAKERBOND® do ekstrakcji wspomaganej cieczą (SLE) są idealną i szybszą alternatywą dla tradycyjnych metod ekstrakcji ciecz-ciecz i umożliwiają doskonałe odzyskiwanie analitów z matryc biologicznych.

Producent: Avantor

Opis: Czynniki na bazie krzemionki stosowane do oczyszczania białek w oparciu o hydrofobowość wywoływaną przez sól różnicową. Tryb mieszany zapewnia wyjątkową selektywność dzięki oddziaływaniom hydrofobowym i funkcji wymiany jonowej.

MSDS

Producent: G-Biosciences

Opis: Streptavidin Resin is designed for the single step small and large scale affinity purification of proteins and antibodies with a biotin tag. Biotin, a 244 Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10¹⁵ M⁻¹) and streptavidin (Ka=10¹⁵ M⁻¹). Biotin and (strept)avidin interaction is rapid and once the bond is established it can survive up to 3M guanidinehydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8M guanidine-hydrochloride at pH 1,5 or by autoclaving. The biotinylated molecules are efficiently probed with avidin or streptavidin conjugated to reporter molecules, such as peroxidases or phosphatases.

Numer katalogowy: (IMMRIR1011)

Producent: ImmunoReagents

Opis: ImmunoReagents Human IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Human IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

j.m.: 1 * 2 mL

Wyprzedaż

Numer katalogowy: (IMMRIR1012)

Producent: ImmunoReagents

Opis: ImmunoReagents Llama IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Llama IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

j.m.: 1 * 2 mL

Wyprzedaż

Producent: Rockland Immunochemicals

Opis: Protein G is a surface protein of two groups of streptococcal bacteria that has ability to bind immunoglobulins and also to albumins. Protein G are prepared from chromatographically pure protein G.

Numer katalogowy: (786-789)

Producent: G-Biosciences

Opis: Immobilised Pepstatin is for the purification of cathepsins, pepsin, bacterial aspartic proteases, HIV proteases and other molecules that bind pepstatin.

j.m.: 1 * 5 mL

Wyprzedaż

Producent: G-Biosciences

Opis: Jacalin, or <i>Artocarpus integrifolia</i> lectin, is a tetrameric two-chain lectin with a molecular weight of 66 kDa. It is a α-D-galactose binding lectin purified from jack fruit (<i>Artocarpus integrifolia</i>) seeds.

Producent: G-Biosciences

Opis: Glutathione resin is designed for the affinity purification of proteins with a glutathione S-transferase (GST) tag. The resin consists of reduced glutathione (GSH) covalently coupled to 4% cross-linked agarose, via a 10-carbon spacer arm. The resin has a binding capacity of ~8 mg GST/ml resin. Supplied as slurry in 20% ethanol.

Numer katalogowy: (IMMRIR1013)

Producent: ImmunoReagents

Opis: ImmunoReagents Mouse IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Mouse IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

j.m.: 1 * 2 mL

Wyprzedaż

Producent: G-Biosciences

Opis: Cobalt chelating resin, 1 ml columns, for the purification of 6X His proteins

Producent: G-Biosciences

Opis: Thiophilic adsorption or thiophilic chromatography is a routinely used technique for the low cost, simple purification of immunoglobulins. Thiophilic adsorption was first developed by Porath et al in 1984 and is a group specific, salt-dependent purification technique that has distinct affinity towards immunoglobulins and α2- macroglobulins. The thiophilic adsorption works on the principle that some proteins in high salt are able to bind to an immobilised ligand that contains a sulfone group in proximity to a thioether group. The bound proteins are then eluted in decreasing salt concentrations. The thiophilic resin binds immunoglobulins, including IgG, IgY and IgM, from serum, ascites or tissue culture supernatants and the purified immunoglobulins are then eluted in a near neutral aqueous buffer.

Producent: G-Biosciences

Opis: This avidin resin is designed for the single step small and large scale affinity purification of proteins and antibodies with a biotin tag. The resin can also be used for immunoprecipitations using biotin labelled antibodies.

Producent: G-Biosciences

Opis: Concanavalin A (Con A) agarose consists of Con A coupled to 6% agarose by the cyanogen bromide method. Con A is a tetrameric metalloprotein lectin isolated from <i>Canavalia ensiformis</i> (jack bean). It is used for the purification of glycoproteins, polysaccharides and glycolipids as it binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. It has also be used in other application areas including purification of enzyme-antibody conjugates, purification of IgM and separation of membrane vesicles. As stated above, it is a metalloprotein and to maintain its binding characteristics the presence of both Mn²⁺ and Ca²⁺ is essential. Each subunit of Con A utilises one calcium and one manganese ion and these cations can be removed under acidic conditions abolishing the carbohydrate-binding activity.