Wyszukano: Materiały edukacyjne z biologii

Numer katalogowy: (BE-404)

Producent: G-Biosciences

Opis: This lab activity is designed to demonstrate the different classes of protein molecules and their classification based on solubility. Students learn fractionation of soluble, insoluble membrane proteins, and cytoskeleton proteins from a tissue sample. The insoluble protein fraction is further fractionated into hydrophilic and hydrophobic membrane proteins. Cell membrane structure and the role of hydrophobic membrane proteins are considered. This lab activity also provides an opportunity to understand characteristics of various classes of detergents and the role of detergents in solubilisation of hydrophobic membrane proteins.

j.m.: 1 * 1 KIT

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Numer katalogowy: (BE-401)

Producent: G-Biosciences

Opis: The physical properties of proteins kit is a lab activity that enables students to investigate the physical properties of several different proteins. Students will learn about protein solubility and how it is affected by various parameters; including temperature, pH, salt and dielectric constant. They will understand about protein precipitation due to pH, high salt and in the presence of organic solvents and about protein denaturation as a result of high temperature. In addition, the kit will demonstrate how non-protein agents, such as detergents drastically alter the physical properties of protein molecules and as a result, understand the importance of detergents in protein solubilisation. This lab activity involves analysis of three different types of pure proteins and then students alter some of those properties with a detergent and re-examine physical properties of those proteins. Students are challenged to consider how physical properties of protein molecules can be exploited for purification and characterisation of proteins and apply their findings on a test sample of complex tissue extract.

j.m.: 1 * 1 KIT

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Numer katalogowy: (BE-502)

Producent: G-Biosciences

Opis: Dot blotting is a simple technique to identify a known protein in a biological sample. The ease and simplicity of the technique makes dot blotting an ideal diagnostic tool.

j.m.: 1 * 1 KIT

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Numer katalogowy: (EDVOS-49)

Producent: EDVOTEK

Opis: Solve the mystery of two boys separated from their parents a decade ago. Their biological mother is identified by mitochondrial DNA and their biological father from chromosomal DNA.

j.m.: 1 * 1 SZT

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Numer katalogowy: (EDVO282)

Producent: EDVOTEK

Opis: This easy and safe experiment allows students to learn about enzyme catalysis, the nature of enzyme action, and protein structure-function relationship. Students will perform an enzyme assay and determine the rate of the enzymatic reaction.

j.m.: 1 * 1 KIT

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Numer katalogowy: (EDVO270)

Producent: EDVOTEK

Opis: This experiment introduces students to the principles of antigen-antibody interactions by using the Ouchterlony procedure. Antibodies and antigens form complexes that precipitate, making it possible to assay antibody-antigen systems. The binding interaction results in the formation of a white precipitate after diffusion in agarose.

j.m.: 1 * 1 SZT

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Numer katalogowy: (EDVO271)

Producent: EDVOTEK

Opis: An HIV test detects HIV infection indirectly using an ELISA test against HIV antibodies in the blood. The test works by taking antibodies from the patient's blood and adding them to a microtitre plate coated with HIV antigen. If HIV antibodies are present, they will bind to the antigens on the plate. In this experiment, students perform an ELISA text by coating microtitre plate wells with simulated HIV antigen and then test simulated donor serum for anti-HIV antibodies.

j.m.: 1 * 1 SZT

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Producent: EDVOTEK

Opis: Plasmid and lambda DNA are pre-digested with restriction endonucleases that recognise and cut double-stranded DNA within or near defined base sequences. Digests are separated by agarose gel electrophoresis.

Numer katalogowy: (EDVO202)

Producent: EDVOTEK

Opis: Small-scale, rapid isolation of plasmid DNA is a routine procedure used for screening and analysis of recombinant DNAs in cloning and sub-cloning experiments. In this experiment, students isolate plasmid DNA without the use of toxic chemicals such as phenol or chloroform.

j.m.: 1 * 1 KIT

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Numer katalogowy: (EDVO212)

Producent: EDVOTEK

Opis: The DNA from bacteriophage Lambda is a well-characterised linear molecule containing six recognition sites for <i>Eco</i> RI (5 distinct sites, 2 are very close in size). In this experiment, Lambda DNA is digested by the <i>Eco</i> RI endonuclease. The digestion products are analysed by agarose gel electrophoresis.

j.m.: 1 * 1 KIT

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Numer katalogowy: (EDVO124)

Producent: EDVOTEK

Opis: This experiment presents a bioterrorism scenario, with students examining a simulated DNA fingerprinting test for the detection of smallpox.

j.m.: 1 * 1 SZT

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Numer katalogowy: (EDVO221)

Producent: EDVOTEK

Opis: In this experiment, students see a blue colour change in transformed cells due to the switching on of a gene. The pGAL plasmid gives a blue colour dye due to the production of the β-galactosidase protein by the <i>lacZ</i> gene. IPTG is not required in this experiment since pGAL contains the complete <i>lacZ</i> gene.

j.m.: 1 * 1 SZT

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Numer katalogowy: (EDVOS-70)

Producent: EDVOTEK

Opis: The body defends itself from attack by infectious agents like bacteria and viruses by producing antibodies. Enzyme linked immunosorbent assays (ELISA) test for antibodies present in the blood, which indicate infection. In this experiment, students perform a simulated ELISA test to identify infected samples and compare them to control samples.

j.m.: 1 * 1 SZT

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Producent: EDVOTEK

Opis: This experiment introduces students to the use of DNA fingerprinting in a simulated paternity determination. A child's DNA fingerprint is compared with his parents.

Numer katalogowy: (EDVO141)

Producent: EDVOTEK

Opis: Cancer cells differ from normal cells by the combinations of proteins that are present on their surfaces. Antibodies against these proteins will specifically bind to cancer cells and not to normal cells. This allows early detection of cancer and potentially a way of delivering cancer therapies. In this simulation experiment the reaction of cancer cell markers and their corresponding antigens are demonstrated.

j.m.: 1 * 1 SZT

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Numer katalogowy: (EDVO222)

Producent: EDVOTEK

Opis: The Green Fluorescent Protein (GFP) from the jellyfish <i>Aequorea victoria</i> is used extensively in all areas of science. Many organisms have been transformed with the GFP gene, the gene responsible for bioluminescence in jellyfish. It has proven to be so useful that scientists have mutated it to produce Blue Fluorescent Protein (BFP). In this simple experiment, students transform bacteria either by GFP, BFP or both.

j.m.: 1 * 1 SZT

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